Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf

Varování

Publikace nespadá pod Fakultu sociálních studií, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

Autoři	JAYASREE Aswathy SALAVA Hymavathi NODZYNSKI Tomasz THULA Sravankumar
Rok publikování	2024
Druh	Kapitola v knize
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
Popis	Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting proteins to fluorescent protein fragments, BiFC allows visualization of spatial localization patterns of protein complexes. This method has been adapted to a variety of expression systems in different organisms and is widely used to study protein interactions in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts is presented.
Související projekty:	Unravelling novel intracellular trafficking regulators involved in plant stress response.