Studies of microbiological quality of preparations with antimicrobial preservatives and therapeutic bacteriophages of the genera Pbunavirus and Kayvirus
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Antimicrobial preservatives can be used to maintain the microbiological quality of phage preparations, but due to their potential antiviral effect, they may have an adverse influence on phage activity. Moreover, there is a possibility of physicochemical incompatibilities between phage particles and the preservatives. These phenomena may disrupt the effectiveness and quality of the final dosage form containing phages as an active ingredient. For this reason, the subject of this study is the compatibility of antimicrobial preservatives with therapeutic phages in liquid dosage form. We evaluated the stability of phage activity after exposure to 12 preservatives selected from various chemical classes by the double-layer agar method. Staphylococcal phage DSM 33473 of the genus Kayvirus and the pseudomonal phage DSM 33593 of the genus Pbunavirus, with a 9 log PFU/ml titer purified into physiological saline solution, were tested. The influence of the phages on the effect of the preservatives was determined by the microdilution method on Escherichia coli CCM 4517, Candida albicans CCM 8215 and Aspergillus brasiliensis CCM 8222. Physicochemical incompatibilities were evaluated by dynamic light scattering analysis. The assessed preservatives were classified into suitable, moderately suitable and unsuitable for manufacturing phage preparations. The suitable preservatives with slight reduction in phage titre were m-cresol, phenylethyl alcohol, sodium benzoate and sodium propionate. The unsuitable were chlorhexidine digluconate, benzalkonium chloride, thimerosal and diazolidinylurea. Chlorhexidine precipitated in the absence of phages, while benzalkonium only in the presence of phages. Thimerosal caused a decrease of 7 log PFU/ml only in DSM 33593. Diazolidinylurea led to a complete loss of titer in both genera. The result of this work is a classification of preservatives that can be used to optimise dosage forms with bacteriophages and ensure that they do not pose risks to patients due to microbial contamination or phage degradation caused by excipients. The study was supported by the National Institute of Virology and Bacteriology (LX22NPO5103) - Funded by the EU - NGEU. The students wish to thank the GA MU for its support (MUNI/A/1603/2024). |
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